DNA sequencing is most frequently used to determine genome sequence. An example of this type of application is the human genome project. Five years after the completion of the human genome project, we are seeing its impacts on biomedical research. Several genes have been identied to associate with genetic conditions, including familial breast cancer and colorectal cancer, Alzheimer's disease, myotonic dystrophy, neurofibromatosis.
Over the past 10 years, R&D budgets in pharmaceutical and biotech companies have steadily increased. The rising cost of bringing a drug to market has created an environment in which frequent blockbuster drugs are needed to sustain the modern pharmaceutical industry. Today, promising drugs are not pursued because they lack in the broad patient efficacy required to achieve this blockbuster status.
This application note describes the high-throughput isolation of genomic DNA from different populations of three-spined stickleback as well as from three-spined stickleback eggs using Invisorb DNA Tissue HTS 96 Kit. The results showed that the extracted genomic DNA can be used for high scale genotyping and genotyping of adaptive and ecological relevant genes.
Researchers had identified three genes that are connected to HNPCC: MLH1, MSH2 and MSH6. Under normal conditions, these genes actually help the body to resist cancers. On the other hand, mutations of these genes will make the body more susceptible to cancer, more specfically, colorectal and endometrial cancers.
